Figure 1. The experimental set-up and dosage optimization.
(A) Schematic description of the method used for the evaluation of the different tRNA scaffolds. (B) Fluorescence recovery versus the amount of suppressor tRNASer transfected. Identical amount of reporter plasmid encoding GFP S29stop is used in all transfections. Fluorescent values reported here are obtained from the average of 4 independent transfections. The dashed line indicates the observed fluorescence when equivalent amount of plasmid encoding wild type GFP is used.