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. Author manuscript; available in PMC: 2011 Feb 26.
Published in final edited form as: J Mol Biol. 2009 Dec 21;396(3):821. doi: 10.1016/j.jmb.2009.12.018

Figure 6. Comparing in vivo stability of high, medium and low efficiency suppressors.

Figure 6

(A) S01, S07 and S15 scaffolds show equivalent stability when incubated in human cells. The 3′ terminal A of corresponding tRNAs has been 32P-labeled using CCA-adding enzyme and lipofected in HeLa cells. Total RNAs have been extracted at different time points and separated on denaturating PAGE. (B) Fraction of radioactive signals for the tRNA band at different time points shows similar half-lives for these suppressor-tRNAs. It corresponds to the ratio of the radioactive signal specific to full-length tRNA over the signal of the entire lane.

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