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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Chem Res Toxicol. 2010 Feb 15;23(2):341. doi: 10.1021/tx9003402

Figure 2. Influence of reaction conditions on DPC formation by Cr(VI).

Figure 2

Standard reaction mixtures contained 5 μg DNA, 60 μg BSA, 25 mM MES (pH 6.0) or 25 mM MOPS (pH 7.0), 1 mM Asc and 0 or 100 μM Cr(VI). Data are means ±SD (n=3). Where not seen, error bars were smaller than symbols. (A) Time-course of DNA-BSA crosslinking. (B) DPC formation as a function of BSA concentration (reaction time =3 hr, MES buffer). (C) Importance of the initial reaction period in the DPC production (0 – 180 min, DNA was added before the start of Cr(VI) reduction; 5 – 180 min, DNA was added 5 min after the start of Cr(VI) reduction).