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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Chem Res Toxicol. 2010 Feb 15;23(2):341. doi: 10.1021/tx9003402

Figure 5. DPC formation using separately produced Cr-DNA and Cr-BSA adducts.

Figure 5

DNA and BSA were separately modified in 30-min long reactions with Cr(III) or mixtures of Cr(VI)-1 mM Asc, purified using BioGel P-30 columns and then incubated for 3 hr with the unmodified component to produce DPC. Data are means ± SD (n=3). If not seen, error bars were smaller than symbols. (A) DPC formation using BSA or DNA modified with Cr(III) or (B) Cr(VI). (C) The presence of small ligands has no effect on DPC formation in reactions of BSA with pre-formed Cr-DNA adducts. DNA was modified with 100 μM Cr(VI)/1 mM Asc for 30 min, purified using BioGel P-30 columns and incubated for 3 hr with BSA in the presence of 2 mM Asc, Cys or GSH. (D) Influence of small ligands on DPC production during Cr(VI) reduction with 1 mM Asc. Ctrl – standard reaction, +Asc – additional 2 mM Asc was added, +Cys – 2 mM Cys was added, +GSH – 2 mM GSH was added.