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. Author manuscript; available in PMC: 2011 Feb 20.
Published in final edited form as: Virology. 2009 Dec 9;397(2):358. doi: 10.1016/j.virol.2009.11.010

Fig. 4.

Fig. 4

Mutations of lysines 70 and 76 and the 'late domain'-like motif in p33 inhibit TBSV accumulation in yeast. (A) Top panel: Northern blot analysis of total RNA extracts obtained from yeast 48 hours after induction of repRNA replication. The given mutated p33 is shown on the top. Middle panel shows the ribosomal RNAs as loading controls for the Northern blot. The graph shows the level of TBSV repRNA accumulation in comparison with wt p33 (100%). Each mutant was tested in eight repeats. Bottom panel shows a Western blot analysis of total protein extracts for measuring p33HF levels in yeast using anti-6×His antibody. (B) The level of TBSV repRNA accumulation in the presence of K70, 76R/PSVP-TS p33 mutant and wt p92 in the parental and Δvps23 yeast. Note that the accumulation level of TBSV repRNA in the presence of wt p33/p92 in the parental yeast was chosen as 100%.

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