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. Author manuscript; available in PMC: 2011 Feb 20.
Published in final edited form as: Virology. 2009 Dec 9;397(2):379. doi: 10.1016/j.virol.2009.11.030

Figure 6.

Figure 6

Shp2 is required for vGPCR-induced migration in BOEC. (A) BOEC were lenti- transduced with control virus LKO or vGPCR with and without lenti-sh4 to knockdown Shp2. After 48 hours cells reached confluence and a scratch “wound” was made with a pipette tip and medium changed to low-serum (0.1% FBS) without additional supplements. The width of the scratch and the number of cells migrating back into the wound were quantified using NIH ImageJ, 10 hours post-wounding (width measured in arbitrary units). (B) Shp2 was inhibited by PHPS1 (10 μM) where indicated for 12 hours prior to wounding. Error bars represent S.D. One representative of 3 experiments is shown.