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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Toxicol Lett. 2009 Dec 16;193(1):33. doi: 10.1016/j.toxlet.2009.12.004

Figure 4. Overexpression of GCL inhibits As3+-induced cytochrome c release and caspase-9 activation.

Figure 4

Hepa-V3 and Hepa-CR17 cells were treated with 20 uM As3+ for the time periods indicated. (A) Cytosolic and mitochondrial fractions were isolated by a digitonin lysis procedure (Single et al., 1998) and cytochrome c expression in the fractions was assessed by immunoblotting. (B) Caspase-9-like activity was measured utilizing the fluorogenic substrate Ac-LEHD-AMC. The data presented in B are averages +/− SEM of at least three experiments. # p < 0.001, indicates a significant difference compared to As3+-induced caspase activity in Hepa-V3 cells at that time point.