Fig. 5.

Full in vivo ³¹P spectra of K. stuttgartiensis converting ammonium nitrite. Acquisition time = 4 h (26,800 spectra), line width = 25 Hz. The three pH related peaks are 1.5 ppm (pH 6.3, assigned to the anammoxosome), 2.9 ppm (shoulder, pH 7.4, assigned to the riboplasm), and 3.1 ppm (pH 7.8, the extracellular pH). Four non-pH peaks are visible at −18.6, −10.8, −5.2, −0.8, 4.1, and 4.5 ppm, which can probably be assigned to β-ATP (−18.6), NAD/NADP (−10.8 ppm), γ-ATP (−5.2), phosphoenolpyruvate (PEP, −0.8 ppm). The peaks at 4.1 and 4.5 ppm are probably a phosphate diesther and a phosphate monoesther, respectively