Table 2.
Back-calculated concentrations from calibration curves over the concentration range of 5 to 1000 nM.a
| Nominal Concentration (nM) | Calculated Concentration (nM)b | Accuracy (%) | Precision (%) |
|
|---|---|---|---|---|
| Within-Run | Between-Run | |||
| 5 | 5.4 ± 0.3 | 107.0 | 5.8 | -c |
| 10 | 10.3 ± 0.6 | 102.7 | 5.7 | -c |
| 50 | 48.2 ± 1.8 | 96.4 | 1.4 | 3.8 |
| 100 | 95.6 ± 5.7 | 95.6 | 7.5 | -c |
| 500 | 467.7 ± 35.6 | 93.5 | 4.1 | 7.2 |
| 1000 | 1037.7 ± 45.4 | 103.8 | 4.3 | 0.5 |
a
Performed in duplicate on 3 separate days.
b
Values are mean ± standard deviation.
c
No significant variation was observed as a result of performing the assay in different runs.