Table 3.

Assessment of accuracy, precision, and recovery^a

Nominal Concentration (nM)	Accuracy (%)	Precision (%)		Recovery (%)
		Within-Run	Between-Run
Plasma
5	99.5	5.5	9.5	-b
8	101.9	6.6	6.7	77.4
80	99.4	4.7	6.7	70.1
800	102.2	3.0	5.4	62.7
Bone Marrow Supernatantc
5	104.1	2.2	8.8	-b
8	100.7	3.2	2.7	92.5
80	100.2	4.4	-d	100.5
800	102.4	4.0	-d	92.0
Bone Marrow Cellse
5	103.8	2.4	6.0	-b
8	97.7	2.7	3.3	104.8
80	95.2	3.0	1.3	97.8
800	101.7	1.8	3.2	100.0

^aPerformed in quintuplicate on three separate days

^bNot done

^cSample diluted 1:1 (v/v) with plasma prior to extraction

^dNo significant additional variation was observed as a result of performing the assay in different runs.

^eSample suspended in 100μL of plasma prior to extraction.