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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Structure. 2010 Jan 13;18(1):127. doi: 10.1016/j.str.2009.10.020

Figure 6.

Figure 6

A role for Asp168 in strand ligation. Asp168 makes short van der Waals or CH-O hydrogen bonding contacts to the −1 and +2 sugars located on opposite faces of the minor groove. The β7-β8 loop is anchored to the upstream DNA via four distinct interactions involving the backbone and side chain of Asp168 and the side chain of Lys167. Lys167 and Asp168 in turn provide a docking surface for the -1 sugar, where interactions with the ribose ring and O5′ may promote a favorable conformation for efficient ligation. Asp168 contacts several ribose ring atoms with distances < 3.5 Å; the closest contacts are shown.