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. Author manuscript; available in PMC: 2010 Feb 16.
Published in final edited form as: Mol Cell. 2010 Jan 15;37(1):5–6. doi: 10.1016/j.molcel.2009.12.028

Figure 1. Regulation of replication licensing by Hbo1.

Figure 1

(A) From late M- to G1-phase ORC, Cdc6 and Cdt1 sequentially load onto chromatin to mark origins of replication.

(B) Cdt1-assisted binding of Hbo1 to origins results in acetylation of histone H4 at origins.

(C) The presence of ORC-Cdc6-Cdt1 and acetylated histones facilitate the loading of Mcm2-7 complex.

(D) Geminin binds to Cdt1 during S- and G2-phases of cell cycle and blocks its interaction with Mcm2-7 as well as inhibiting Hbo1 mediated H4 acetylation thus preventing re-loading of Mcm2-7 once replication is initiated.