Skip to main content
. Author manuscript; available in PMC: 2010 Feb 16.
Published in final edited form as: J Biol Chem. 2001 Nov 21;277(6):4317. doi: 10.1074/jbc.M109739200

Fig. 5. A Western blot illustrating the effect of PAO on the tyrosine phosphorylation level of ROMK1 in the presence and absence of PAO.

Fig. 5

The cells were treated with PAO or vehicle for 15 min. The ROMK1 channels were harvested by immunoprecipitation (IP) of the cell lysate with GFP antibody. The phosphorylated ROMK1 was detected with PY20 (left panel), and the total ROMK1 is recognized by ROMK antibody (right panel). The protein phosphorylation level is normalized by comparison to the relative amount of the total ROMK1 protein. IB, immunoblotting.