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. Author manuscript; available in PMC: 2010 Feb 16.
Published in final edited form as: J Biol Chem. 2001 Nov 21;277(6):4317. doi: 10.1074/jbc.M109739200

Fig. 7. A Western blot shows the effect of PAO on the membrane fraction of ROMK1 or R1Y337A labeled by biotin.

Fig. 7

The cells transfected with ROMK1/R1Y337A + c-Src were treated with PAO for 15 min followed by biotin labeling at 4 °C. The ROMK1/R1Y337A proteins were harvested by immunoprecipitation (IP) of cell lysate with GFP antibody. The membrane fraction of the K+ channels was detected with neutravidin horseradish peroxidase (left panel), and the total ROMK1/R1Y337A proteins were identified with ROMK antibody (right panel). The data were normalized according to the total K+ channel protein level. IB, immunoblotting.