Fig. 5.

GLP-2 dose-dependently stimulated glucose uptake by hippocampal neurons. a: primary neurons were traced while taking up glucose directly using fluorescent nonmetabolized glucose analog 6-NBDG {6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose} imaging method. b: protein of GLUT3 (predominant isoform of facilitative GLUTs) was expressed in hippocampal neurons cultured on DIV 5, where neurons were identified by neuronal marker PGP9.5 (in red) and GLUT3 immunostained (in green). c: glucose uptake was quantified by 2-deoxy-d-[³H]glucose ([³H]2-DG) uptake assay. Approximately 96.5% of 2-DG uptake was attributed to cytochalasin B-sensitive, i.e., mainly through facilitative GLUT-mediated uptake. d: glucose uptake was stimulated in a dose-dependent manner by GLP-2 treatment for 30 min and maximized at 20 nM. Experiments were repeated independently 3 times. *P < 0.05 and **P < 0.01.