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. 2009 Nov 17;298(2):E304–E319. doi: 10.1152/ajpendo.00504.2009

Fig. 1.

Fig. 1.

Wild-type (WT) estrogen receptor (ER)α transcript and protein are reduced in insulin target tissues from ERα-knockout (KO) mice. ERα mRNA/β-actin expression in skeletal muscle (A), liver (B), and adipose tissue (C) harvested from normal chow (NC)-fed WT (open bars; n = 12) and KO (black bars; n = 12) mice following a 6-h fast. WT expression levels were normalized to 1.0. Full-length (66 kDa) ERα protein is undetectable in skeletal muscle (D) and liver (E) from KO mice compared with WT. Representative blots are shown for ERα as well as loading controls, β-actin, and heat shock protein (HSP)90 (n = 5–6 mice/genotype). ERβ mRNA in skeletal muscle (F), liver (G), and adipose tissue (H) from WT (open bars; n = 12) and KO (black bars; n = 12) mice. No differences in ERβ mRNA/β actin expression levels were detected between the genotypes. I: ERα is the predominant ER transcript found in skeletal muscle, liver, and adipose tissue. Values for tissue RT-PCR and immunoblot (IB) analyses are represented as means ± SE and expressed in arbitrary units (AU). Mean differences were detected using ANOVA. *P < 0.05.