Fig. 6.

Whole body ERα deletion leads to adipose tissue inflammation and reduced adiponectin expression. A: IB and densitometric analyses were performed on periovarian white adipose tissue samples harvested from NC-fed WT (open bars) and ERα-KO (black bars) mice to assess the inflammatory marker p-JNK. RT-PCR analyses were performed to determine monocyte chemoattractant protein-1 (MCP-1; B), TNFα (C), leptin (D), and adiponectin expression levels (E). F: reduced adipose tissue adiponectin total protein in KO mice was confirmed by immunoblotting. Values are expressed as means ± SE in AU. Mean differences between genotypes were detected using ANOVA. *P < 0.05.