Fig. 7.

Whole body ERα deletion causes susceptibility to high-fat diet (HFD)-induced glucose intolerance and insulin resistance. GTT (1,000 mg/kg) and euglycemic hyperinsulinemic clamps (6-h fast, 12 mU · kg⁻¹ · min⁻¹ insulin infusate) were performed on WT and ERα-KO mice at 26 wk of age following consumption of a HFD (45% calories from fat) for 8 wk. A: mean blood glucose concentrations ± SE during the GTT are shown for both WT (☐; n = 6) and KO (■; n = 6). Repeated-measures ANOVA with Tukey's post hoc comparison was performed to detect mean differences between the 2 genotypes over time. *P < 0.05. Skeletal muscle insulin sensitivity as represented by IS-GDR (B) and hepatic glucose production (HGP %suppression; C) is expressed as means ± SE for WT (open bars; n = 8) and KO (black bars; n = 6) mice. Mean differences were detected using ANOVA. *P < 0.05.