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Effect of pioglitazone on cell proliferation and oxidative stress markers in β-cells. Representative immunostaining for proliferative cell nuclear antigen (PCNA; A), Ki67 (B), and 4-hydroxy-2-noneal modified protein (4-HNE; C) in pancreatic tissue sections. Bars = 100 μm. D–F: PCNA, Ki67, and 4-HNE expression were analyzed semiquantitatively. Open bars, control group; filled bars, pioglitazone group. Data are means ± SE for 5 mice/group. *P < 0.05.
