Fig. 5.

S711 phosphorylation is controlled by 1 or more of the TBC1D4-4P sites. Gene electrotransfer of tibialis anterior muscles from ICR mice in vivo was used to express TBC1D4-WT, TBC1D4-S711A, and TBC1D4-4P and the mice were studied 7 days later. A: mice (n = 4) were stimulated with AICAR (0.5 mg/g body wt subcutaneously, 30 min) or saline (subcutaneous, 30 min). Muscle lysates were prepared followed by Western blot analysis using phosphospecific antibodies as indicated in the figure. B: tibialis anterior muscles (n = 6) were stimulated to contract in situ (15 min; train rate: 1/s; train duration: 500 ms; pulse rate: 100 Hz; duration: 0.1 ms at 1–10 V) via the common peroneal nerve or sham-operated and left in the rested state (15 min). **Treatment effect (P < 0.01); ††effect of the DNA construct expressed compared with WT (P < 0.01).