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. 2009 Nov 4;298(2):C342–C354. doi: 10.1152/ajpcell.00336.2009

Fig. 7.

Fig. 7.

Quantitative real-time PCR analysis of Prdx6−/− and Prdx6+/+ LEC mRNA showing expression of Tm1α, Tm2β, Vimentin, Uqcrcp1, MrpS22, Txndc5, Serpinb6a, and TGF-β1. Total RNA was isolated and transcribed into cDNA. Real-time PCR was conducted with specific primers corresponding to genes (see materials and methods). mRNA expression of each gene was adjusted to the 18S ribosomal RNA. Results showed that Tm1α, Tm2β, Vimentin, Uqcrcp1, MrpS22, and TGF-β1 mRNA were upregulated and Txndc5 and Serpinb6a mRNA were downregulated in Prdx6−/− LEC. *P < 0.000, **P < 0.0008, ***P < 0.005, †P < 0.05, ‡P < 0.001.