Table 1.
2D-DIGE Sequence |
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---|---|---|---|---|---|---|
Spot No. | Name | Average Ratio | Accession No. | Mr, kDa | Coverage % | Molecular Function |
910* | Tropomyosin (Tm)2β | 6.354↑ | gi:11875203 | 32.931 | 19.0 | Cytoskeleton |
1374 | Vimentin | 6.085↑ | gi:2078001 | 53.69 | 31.2 | Cytoskeleton |
1479 | Vimentin | 6.194↑ | gi:2078001 | 53.69 | 29.4 | Cytoskeleton |
1429 | Ubiquinol-cytochrome c (Uqcrcl) | 1.660↑ | 53.42 | 27.5 | Metalloprotease | |
Reductase complex core protein | gi:14548301 | |||||
1708 | Mitochondrial ribosomal protein S22 (MrpsS22) | 1.563↑ | gi:12963591 | 38.475 | 29.7 | Unclassified |
1778 | Hypothetical protein | 1.958↑ | gi:83010429 | 16.539 | ||
422 | ALG-1 interacting protein | 0.625↓ | gi:4633515 | 96.661 | 16.0 | Select calcium binding protein |
933 | Lamin C | 0.583↓ | gi:1794160 | 65.464 | 19.0 | Intermediate filament |
1126 | Tubulin-specific chaperone e | 0.416↓ | gi:31543843 | 59.790 | 16.6 | Chaperone |
1499 | Thioredoxin domain containing 5 (Txndc5) | 0.597↓ | gi:83921612 | 36.631. | 49.8 | Isomerase |
1511 | Serine (cysteine) protease inhibitor, clade B, member 6a (Seprinb6m) | 0.523↓ | gi:6678097 | 42.913 | 19.6 | Protease inhibitor |
1557 | Gelsolin-like capping protein | 0.564↓ | gi:110227377 | 39.030 | 28.7 | Cytoskeleton |
2266 | Peroxiredoxin6 (PRDX6) | 0.088↓ | gi:3219774 | 24.969 | 61.6 | Oxidoreductase |
LEC, lens epithelial cell; 2D-DIGE, 2-dimensional difference gel electrophoresis; Mr, molecular weight ratio.
Identified on small gel (7 × 7 cm).