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. 2009 Nov 11;298(2):C283–C297. doi: 10.1152/ajpcell.00444.2009

Fig. 10.

Fig. 10.

Xenopus oocytes expressing AE1 E758K exhibit small currents sensitive to the presence of an ectoepitope tag, as recorded by two-electrode voltage clamp. A: Xenopus oocytes expressing wild-type AE1 display basal currents insensitive to bath substitution of Cl− or Na+. B: AE1 E758K-expressing oocytes exhibit a modest, outwardly rectifying current in NaCl bath that is preserved in NMDG Cl but largely abolished in Na gluconate. *P < 0.01, Na gluconate vs. NaCl. C: oocytes expressing HA-tagged wild-type AE1 display basal currents insensitive to bath substitution of Cl− or Na+. D: oocytes expressing HA-tagged AE1 E758K exhibit a modest, inwardly rectifying current in NaCl bath that is preserved, if not enhanced, in Na gluconate but reduced in NMDG Cl. *P < 0.001, NaCl vs. NMDG Cl. All oocytes coexpressed GPA (2 ng cRNA). Values are means ± SE for number of oocytes shown in parentheses. In all panels, NaC1 bath currents are presented as open circles, Na gluconate bath currents as filled circles, and NMDG C1 bath currents as open triangles.