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. 2009 Nov 11;298(2):C283–C297. doi: 10.1152/ajpcell.00444.2009

Fig. 2.

Fig. 2.

Human AE1 E758K mediates glycophorin A (GPA)-dependent Cl−/Cl− exchange. A: effect of DIDS (200 μM) on GPA-dependent 36Cl− uptake by Xenopus oocytes expressing wild-type AE1, AE1 mutant E758K, or E758K with a hemagglutinin (HA) tag in the 3rd extracellular loop [E758K(HA)]. H2O-corrected values are means ± SE for (n) oocytes. *and gray bar indicate P < 0.05 vs. E758K or E758K(HA) coexpressed with GPA (Student‘s unpaired t-test). B: 36Cl− uptake by Xenopus oocytes expressing wild-type AE1 and AE1 mutants. *and gray bar indicate P < 0.01 vs. AE1 (Dunnett’s t-test). C: representative traces of 36Cl− efflux from Xenopus oocytes expressing wild-type AE1 or AE1 mutant E758K(HA), without or with coexpressed GPA, into ND-96 or Cl−-free bath. D: summary of data from experiments in C. E: 36Cl− uptake in Ambystoma mexicanum oocytes expressing wild-type AE1 or AE1 mutants E758K or E758K(HA) in the absence and presence of coexpressed GPA. H2O-corrected values are means ± SE for (n) oocytes. *P < 0.01 vs. +GPA (Student's unpaired t-test).