Fig. 6.

Surface expression of AE1 mutant E758K in Ambystoma oocytes is increased by coexpression of GPA. A and B: confocal immunofluorescence micrographs of HA antigen surface expression by Ambystoma oocytes expressing HA-tagged AE1 mutant E758K (10 ng cRNA) in the absence (A) and presence (B) of coexpressed GPA (2.5 ng of cRNA). C: H₂O-injected oocyte. Scale bar, 100 μm. D: surface expression of AE1 E758K measured as (water-injected oocyte-corrected) mean relative fluorescence intensities ± SE for (n) oocytes; *P < 0.05 compared in the absence (gray bar) and presence (black bar) of GPA (Student's unpaired t-test).