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. 2009 Nov 11;298(2):C263–C273. doi: 10.1152/ajpcell.00308.2009

Fig. 6.

Fig. 6.

Removal of the COOH terminus, residues 79–129, of KCNE1 reduces the level of coimmunoprecipitation with KCNQ1-GFP. Coimmunoprecipitation of 3XFlag-tagged-KCNE1, 3XFlag-tagged-KCNE1 and the LQT5 mutants, G52R and T58P/L59P, with the COOH terminus removed (3XFlag-KCNE1Δ79−129, 3XFlag-G52RΔ79−129, and 3XFlag-T58P/L59PΔ79−129, respectively) with KCNQ1-GFP. A, top: 3XFlag-tagged-KCNE1, ΔC-term79−129-KCNE1, and ΔC-term79−129-LQT5 mutant expression in HEK-293 cell lysate. A, bottom: 3XFlag-tagged-KCNE1, ΔC-term79−129-KCNE1, and ΔC-term79−129-LQT5 mutants that coimmunoprecipitated with KCNQ1-GFP. B, top: KCNQ1-GFP expression in HEK-293 cell lysate. B, bottom: KCNQ1-GFP immunoprecipitation. The Co-IP study shown in A and B is representative of three experiments. C: panels displaying varying lengths of exposure of the Co-IP panel displayed in A. The gel order is the same as that shown for A and B. D: deletion of the COOH terminus of KCNE1 reduces the relative level of association with KCNQ1-GFP. The relative level of association of 3XFlag-KCNE1Δ79−129 in comparison to KCNE1 was determined from three separate experiments. Data are displayed as a percentage of the level seen for KCNE1 and are presented as means ± SE. *Significantly (P < 0.05) different from the value seen for control (KCNQ1-GFP + 3XFlag-KCNE1).