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H-K-ATPase-mediated pHi recovery in microperfused mouse collecting duct (CD). Cell-averaged pHi recovery (peritubular BCECF-AM loading) in the presence of EIPA and bafilomycin A1 was measured in CD segments from cortex (CCD) and outer and inner medulla (OMCD and IMCD, respectively). CCD exhibited the highest rates of pHi recovery. Thick solid line, CCD; dashed line, OMCD; thin solid line, IMCD. *P < 0.05 vs. CCD. **P < 0.05 vs. OMCD.
