Table 1.
Solutions
| Solution |
||||
|---|---|---|---|---|
| A | B | C | D | |
| NaCl | 119.2 | 79.2 | 119.2 | 0 |
| Na gluconate | 0 | 0 | 0 | 119.2 |
| KCl | 3 | 3 | 3 | 0 |
| HEPES | 25 | 25 | 0 | 0 |
| KH2PO4 | 2 | 2 | 2 | 2 |
| CaCl2 | 1.2 | 1.2 | 1.2 | 1.2 |
| Ca gluconate | 0 | 0 | 0 | 4 |
| MgSO4 | 1 | 1 | 1 | 1 |
| Alanine | 5 | 5 | 5 | 5 |
| Glucose | 8.3 | 8.3 | 8.3 | 8.3 |
| NH4Cl | 0 | 40 | 0 | 0 |
| NaHCO3 | 0 | 0 | 25 | 25 |
| Na acetate | 0 | 0 | 1 | 1 |
Solutions C and D were gassed with 95% O2-5% CO2 to pH 7.4 at 37°C and delivered to the bath chamber via a closed-syringe delivery system (PHD2000 infusion syringe pump, Harvard Apparatus, Holliston, MA) with polyethylene tubing (Intramedic, Becton Dickinson, Franklin Lakes, NJ). Under these conditions, there is no significant pH change from the delivery syringe to the perfusion chamber as verified by a bath pH probe (Accumet 13-620-96).