Table 2.
Effect of Sch-28080 and ouabain on βi, pHi recovery, and JH in A- and B-type ICs of the CCD in the presence of EIPA and luminal bafilomycin A1
| N(n) | βi | pHi Recovery, U/min | JH, mM H/min | |
|---|---|---|---|---|
| A-type ICs | ||||
| Control | 29(6) | 74.33 ± 3.85 | 0.30 ± 0.03 | 23.36 ± 2.91 |
| Sch-28080 | 38(5) | 73.36 ± 4.87 | 0.22 ± 0.02† | 15.06 ± 1.25* |
| Ouabain | 35(5) | 56.92 ± 3.07* | 0.21 ± 0.02† | 10.86 ± 1.18* |
| B-type ICs | ||||
| Control | 25(6) | 72.68 ± 7.48 | 0.26 ± 0.03 | 18.68 ± 2.75 |
| Sch-28080 | 22(3) | 63.75 ± 4.60 | 0.12 ± 0.28* | 5.97 ± 1.18* |
| Ouabain | 25(4) | 41.34 ± 2.97* | 0.12 ± 0.03* | 5.03 ± 1.32* |
Values are means ± SE; N, number of cells; n, number of tubules. ICs, intercalated cells; pHi, intracellular pH; CCD, cortical collecting duct; βi, intrinsic buffering capacity; JH, H secretion rate. All comparisons are within the same cell type.
*
P < 0.01 vs. control.
†
P < 0.02 vs. control.