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. 2009 Dec 9;298(2):F323–F334. doi: 10.1152/ajprenal.00307.2009

Fig. 1.

Fig. 1.

Activation of human αβγ-epithelial Na+ channels (ENaC) by 8-(4-chlorophenylthio)-cGMP (8-pCPT-cGMP) in Xenopus oocytes. 8-pCPT-cGMP Na salts and amiloride (Amil) were continuously perfused on oocytes. Whole cell currents were monitored at 10-s intervals. A: representative inward and outward current traces in the presence of 8-pCPT-cGMP (CPT-cGMP) and amiloride. Currents were digitized at both −100 and +70 mV. Application of drugs is indicated by solid horizontal lines. B: average currents at −100 mV (means ± SE). *P < 0.05 compared with basal level. n = 6. C: representative current trace showing no effect of cell-impermeable standard cGMP (Std cGMP). Amiloride was applied at the end of experiments. D: corresponding average currents at −100 mV (means ± SE). E: application of 8-pCPT-cGMP ranged from 0.1 to 1,000 μM. Current trace was recorded at −100 mV. F: dose-response curve. The dashed line was created by fitting the raw data with the Hill equation. n = 10.