Fig. 3.

8-pCPT-cGMP-activated currents associated with human αβγ-ENaC in oocytes perfused with alkali ions. To ascertain that the 8-pCPT-cGMP-activated current was associated with αβγ-ENaC, the effects of 8-pCPT-cGMP (CPT-cGMP) were examined when oocytes were bathed in LiCl and KCl solutions. A and B: representative whole cell current traces elicited in LiCl and KCl media, respectively. Bath solution was switched to LiCl or KCl solution from regular ND-96 medium containing 96 mM NaCl. 8-pCPT-cGMP was perfused to the bath followed by the perfusate with amiloride. C and D: summary of corresponding inward currents at −100 mV. **P <0.01. E and F: summary of corresponding outward currents at +70 mV. *P < 0.05. n = 3.