Fig. 5.

Acute and chronic effects of kinase blocker cocktail on the 8-pCPT-cGMP-mediated stimulation of αβγ-ENaC activity. A: current trace from an oocyte perfused with 8-pCPT-cGMP (CPT-cGMP) and followed by the kinase inhibitor cocktail (PKA inhibitor, Rp-cAMP 100 μM; PKC inhibitor, acryriarubin A 2 μM; MEK inhibitor, PD98059 20 μM; and p38 MAP inhibitor, SB-202190 30 μM). 8-pCPT-cGMP (CPT-cGMP) and the kinase inhibitor cocktail were washed out with ND-96 medium before addition of amiloride. B: pretreatment of oocytes with kinase blocker cocktail. Cells were incubated with the kinase blocker cocktail for 1 h at room temperature, and then the responses of ENaC channels to 8-pCPT-cGMP and amiloride were recorded. Data are represented as means ± SE; n = 4–6. *P < 0.05, **P <0.01.