Fig. 9.
Stimulatory effects of 8-pCPT-cGMP and 8-CPT-cAMP on human αβγ-ENaC single-channel activity. A: representative outside-out recording. The patch potential was −60 mV. Closed (0) and open (1–3) levels were marked. Perfusion of 8-CPT-cAMP (CPT-cAMP, 0.2 mM) and subsequently 8-pCPT-cGMP (CPT-cGMP, 0.2 mM) and amiloride (Amil, 10 μM) was indicated. B: expanded segments for basal (a), 8-CPT-cAMP activated (b), and 8-pCPT-cGMP activated (c) current traces. C: single-channel activity (NPo). *P < 0.05 compared with basal or washout level using paired t-test.