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. 2009 Nov 4;298(2):F293–F300. doi: 10.1152/ajprenal.00410.2009

Fig. 2.

Fig. 2.

Effects of SAHA and TSA on long-term survival of RPTCs following cisplatin treatment. A, C: RPTCs were pretreated for 6 h without or with 5 μM SAHA and then incubated for 18 h with 20 μM cisplatin or 20 μM cisplatin plus 1 μM SAHA. The cells were then changed to fresh culture medium for 24 h of recovery. Cell morphology was recorded by phase-contrast microscopy (A) and whole cell lysate was collected to determine the amount of recovered protein in each condition (C). B, D: RPTCs were pretreated for overnight without or with 1 μM SAHA or 0.1 μM TSA and then incubated with 20 μM cisplatin or 20 μM cisplatin plus 1 μM SAHA or 0.1 μM TSA for 24 h. The cells were then changed to fresh culture medium for 24 h of recovery to record cell morphology (B) and collect whole cell lysate to determine the amount of recovered protein (D). Data in C and D are expressed as means ± SD (n = 3). *Statistically significantly different from the control. #Statistically significantly different from the cisplatin-alone group.