Fig. 6.

SAHA enhances cisplatin-induced apoptosis in HCT116 colon cancer cells. HCT116 cells were pretreated for 6 h without or with 5 μM SAHA and then incubated for 4 or 18 h with 80 μM cisplatin or 80 μM cisplatin plus 1 μM SAHA. A: cell morphology. B: cells were evaluated by morphology to determine the percentage of apoptosis. Data are expressed as means ± SD (n = 3). *Statistically significantly different from control. #Statistically significantly different from the cisplatin-only group. C: after treatment, the cells were fractionated into cytosolic and mitochondrial fractions for immunoblot analysis of cytochrome c.