Table 1.
Cytokine profiles
| Control |
HIF1αΔ/Δ |
|||||
|---|---|---|---|---|---|---|
| Saline | 30 μg | 60 μg | Saline | 30 μg | 60 μg | |
| IL-1β | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.40 ± 0.29 | 7.60 ± 3.91† | 0.00 ± 0.00‡ | 0.00 ± 0.00‡ |
| IL-2 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| IL-4 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 2.71 ± 1.44‡** | 0.00 ± 0.00 |
| IL-5 | 0.00 ± 0.00 | 2.33 ± 1.67 | 0.20 ± 0.20 | 18.30 ± 10.61† | 3.00 ± 1.21‡ | 0.00 ± 0.00‡ |
| IL-6 | 1.80 ± 0.81 | 1.95 ± 0.97 | 5.00 ± 2.53 | 3.81 ± 2.38 | 3.04 ± 2.06 | 5.75 ± 2.18 |
| IL-10 | 0.00 ± 0.00 | 0.10 ± 0.06 | 0.10 ± 0.06 | 0.00 ± 0.00 | 0.39 ± 0.22 | 0.13 ± 0.06 |
| IL-12 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 3.10 ± 1.12† | 1.86 ± 0.83 | 1.58 ± 0.76 |
| IL-13 | 1.00 ± 0.22 | 0.33 ± 0.11 | 1.17 ± 0.21 | 1.00 ± 0.32 | 0.79 ± 0.18 | 1.00 ± 0.22 |
| Eotaxin | 3.75 ± 0.55 | 3.13 ± 0.80 | 2.13 ± 0.52 | 4.25 ± 0.29 | 2.96 ± 1.04 | 2.96 ± 0.88 |
| GM-CSF | 0.00 ± 0.00 | 1.92 ± 0.99 | 2.33 ± 1.02 | 5.40 ± 3.48† | 2.29 ± 0.52 | 1.17 ± 0.65 |
| IFNγ | 0.85 ± 0.40 | 1.80 ± 0.63 | 2.13 ± 0.58 | 2.50 ± 1.31 | 1.71 ± 0.53 | 1.50 ± 0.36 |
| KC | 31.10 ± 8.40 | 46.10 ± 13.80 | 42.58 ± 8.59 | 59.38 ± 6.02 | 37.58 ± 7.53 | 118.75 ± 33.82‡** |
| MCP1 | 0.00 ± 0.00 | 1.30 ± 0.58 | 2.83 ± 1.49 | 2.00 ± 0.71 | 0.83 ± 0.31 | 2.83 ± 1.08 |
| RANTES | 3.00 ± 1.08 | 25.25 ± 7.61 | 47.75 ± 21.39 | 8.50 ± 3.07† | 17.07 ± 6.31 | 18.08 ± 3.04 |
| TNFα | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 24.05 ± 12.47† | 0.29 ± 0.29‡ | 0.00 ± 0.00‡ |
| MIP2 | 45.40 ± 9.72 | 65.67 ± 13.96 | 75.17 ± 27.54 | 82.63 ± 28.57 | 30.83 ± 5.53 | 90.67 ± 29.76 |
| VEGF | 370.20 ± 16.36 | 383.50 ± 56.75 | 349.20 ± 16.56 | 624.00 ± 138.97† | 399.93 ± 44.04‡ | 538.25 ± 43.06** |
| MIP1a | 3.40 ± 1.98 | 5.00 ± 1.75 | 1.30 ± 0.72 | 5.50 ± 1.59 | 1.92 ± 1.18 | 8.83 ± 2.39** |
Cytokine levels in BALF from cobalt-challenged control and HIF1αΔ/Δ mice. †Significance at 0.05 when compared with saline control between genotypes. ‡Significance at 0.05 when compared with saline-treated mice within genotype. **Significance at 0.05 when compared between genotypes and within treatment group. Control and HIF1α Δ/Δ mice were exposed to saline or 30 or 60 μg of CoCl2 for 2 wk, 5 days/wk paradigm. The levels of cytokines in BALF were assessed using the Bio-Rad Bio-Plex cytokine suspension array system. N >5 mice/group. † P < 0.05, when HIF1αΔ/Δ saline is compared with control saline. ‡ P < 0.05 compared with genotype-matched control. ** P < 0.05 when similar treatments are compared between genotypes.