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. 2009 Nov 13;298(2):L252–L260. doi: 10.1152/ajplung.00191.2009

Fig. 2.

Fig. 2.

The PcCdc42 homolog is present on a single chromosome within the Pneumocystis genome. A 576-bp PcCdc42 probe hybridized under high-stringency conditions to a single Pc chromosome. Samples of Pneumocystis chromosomes resolved by contour-clamped homogeneous electrical field electrophoresis (CHEF), with each lane representing the total Pneumocystis organisms derived from a single infected rat (i.e., 10 separate rat lung samples). Left: ethidium bromide-stained CHEF gel shows separated samples of Pneumocystis chromosomes. Right: hybridization of blot of PcCdc42 probe to separated samples of Pneumocystis chromosomes transferred to nitrocellulose.