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Steady-state PcCdc42 mRNA appears to be expressed equally in both major life cycle forms of the organism. To examine whether PcCdc42 expression is differentially regulated over the life cycle of P. carinii, organisms were separated into cystic and trophic populations, and total RNA was isolated. Top shows hybridization of the PcCdc42 probe, whereas the bottom shows repeat hybridization of the membrane with Pneumocystis actin to confirm equal loading.
