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. 2009 Nov 13;298(2):L252–L260. doi: 10.1152/ajplung.00191.2009

Fig. 5.

Fig. 5.

PcCdc42 restores growth by complementation of a temperature-sensitive cdc42Δ yeast strain. The full-length cDNA of PcCdc42 was subcloned into pYES2.1 TOPO under the control of the GAL1 promoter. After selection of transformants on selective medium with 2% glucose, single colonies were transferred on selective media containing either 2% glucose (noninducing) (A, B) or 2% galactose (C, D) to induce the GAL1 promoter. On all plates, orientation of yeast is as follows: top, left: ScCdc42Δ + pYES2.1 TOPO/ScCdc42 cDNA; top, right: ScCdc42Δ + pYES2.1 TOPO/PcCdc42 cDNA; bottom, middle: ScCdc42Δ + pYES2.1 TOPO/lacZ control vector.