Fig. 6.
Expression of PcCdc42 protein and its GTP binding and GTPase activity in the presence and absence of Clostridium difficile toxin B (CDTB). A: Western blotting of pET102/D-TOPO/PcCdc42 (lane 1) and LacZ (lane 2) expressed by the RTS 100 E. coli HY kit. B: the purified PcCdc42 protein was assayed for 35S-GTP binding in the presence and absence of CDTB. LacZ demonstrates absence of GTP binding of this irrelevant protein expressed by the same RTS 100 E. coli HY kit. C: intrinsic GTPase activity of PcCdc42 protein in the presence and absence of CDTB.