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HK stimulated mRNA synthesis of TF in mononuclear cells. Mononuclear cells were incubated with HKa or LPS for the indicated time. Total RNA of the cells was isolated by TRIzol reagent. RT-PCR was carried out as described in materials and methods. PCR products were separated in 1% agarose gel. The detection of the 588-bp DNA bands indicated TF mRNA synthesis from corresponding background (0 time point).
