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. 2010 Feb 15;207(2):417–427. doi: 10.1084/jem.20092449

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© 2010 Boboila et al.

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Figure 3. — Ku70- and Ku70/Lig4-deficient cells undergo robust CSR to IgG3 and IgG2b. (a) Representative FACS panel for IgG3 CSR in B cells stimulated with LPS and anti–IgD-dextran and assayed at day 3.5. Data shown are representative of >10 experiments, with one or more mouse of each genotype per experiment. Table S1 contains a summary of all FACS experiments. (b) ELISA quantification of IgM, IgG3, and IgG2b secretion (micrograms/milliliter) in B cells stimulated as in a. Black circles, H/H (the knock-in prerearranged heavy chain receptor is present on both alleles); white circles: H/+ (knock-in heavy chain on one allele). Each circle represents one mouse. All experiments completed are shown, with one mouse per genotype per experiment. Table S2 contains a summary of all ELISA experiments. (c) Representative ELISPOT assay on B cells stimulated as in A. (top) Dots represent Igκ- and Igλ-secreting B cells. (bottom) Dots represent IgG3-secreting B cells. Percentages of B cells undergoing CSR to IgG3 are shown. Data shown are representative of three experiments, with one mouse of each genotype per experiment.