Table I.

Analysis of T cell populations in Cre/PLCγ1^fl/− and the control mice

		Thymocytes					Splenocytes
		Total	DN	DP	CD4 SP	CD8 SP	Total	CD4+	CD8+
PLCγ1+/− (n = 6)	%		1.5 ± 0.8	87 ± 2.8	8.5 ± 1.5	2.7 ± 1.2		22 ± 3.6	15 ± 2.9
	# (×106)	216 ± 76	3.2 ± 2.0	189 ± 69	19 ± 8.3	6.4 ± 4.1	100 ± 46	22 ± 11	15 ± 6.6
PLCγ1fl/− (n = 6)	%		1.1 ± 0.7	87 ± 4.1	9.4 ± 2.1	2.8 ± 1.5		18 ± 8.7	12 ± 5.1
	# (×106)	238 ± 61	2.6 ± 1.5	223 ± 40	24 ± 6.4	6.9 ± 3.4	93 ± 42	17 ± 11	11 ± 7.6
Cre/PLCγ1+/− (n = 8)	%		2 ± 0.8	87 ± 1.5	8.3 ± 1.5	2.9 ± 1.0		15 ± 5.3	12 ± 4.0
	# (×106)	201 ± 88	3.6 ± 1.5	174 ± 72	17 ± 8.3	6.0 ± 4.0	118 ± 46	18 ± 8.6	13 ± 6.6
Cre/PLCγ1fl/− (n = 9)	%		2.1 ± 1.2	95 ± 2.3a	1.5 ± 0.43a	1.2 ± 0.9b		3.2 ± 1.5a	5.6 ± 1.8c
	# (×106)	172 ± 81	3.7 ± 2.8	170 ± 72	2.7 ± 1.7c	2.3 ± 2.0d	77 ± 27	2.7 ± 2c	4.1 ± 2.8b

The age of the mice analyzed was between 3 and 10 wk. Data presented are average percentage (%) or absolute number (#) of each T cell subset. The P value was calculated by comparing the percentages or absolute numbers of different T cell subsets from Cre/PLCγ1^fl/− mice to those from Cre/PLCγ1^+/− mice. There was no significant difference in the percentages and absolute numbers of different T cell subsets from PLCγ1^+/−, PLCγ1^fl/−, and Cre/PLCγ1^+/− mice.

^aP < 0.0001.

^bP < 0.01.

^cP < 0.001.

^dP < 0.05.