Figure 1.

Human CD127⁺ LTi-like cells are precursors of CD56⁺CD127⁺ cells and have similar phenotype to NK22 cells. (A) Lin⁻, CD56⁺ cNK, CD56⁺CD127⁺, or CD127⁺ LTi-like cells, were sorted from tonsil and were co-cultured with human MSCs for 4 d with 10 ng/ml IL-7. Expression of ICAM1 and VCAM1 on MSCs was quantified by flow cytometry. (B) Expression of the indicated molecules was quantified using flow cytometry on Lin⁻ tonsil cells. (C) Flow cytometry–sorted CD127⁺ LTi cells were labeled with CFSE and stimulated with IL-7, IL-2, or IL-15 for 6 d, followed by flow cytometry to assess CD56 expression. One donor out of at least two analyzed is shown in A, B, and C.