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. 2010 Feb 15;207(2):281–290. doi: 10.1084/jem.20091509

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© 2010 Crellin et al.

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Figure 3. — Heterogeneous cytokine production from expanded and clonal CD127⁺ LTi-like cells. (A) Cell lines were stimulated with PMA and ionomycin for 24 h, supernatants were collected, and cytokine production was analyzed by Luminex assays and ELISA. (B) Intracellular cytokine analysis of expanded cNK and CD127⁺LTi-like cell lines, stimulated for 6 h with PMA and ionomycin. (C and D) Intracellular cytokine analysis of LTi cell clones, stimulated for 6 h with PMA and ionomycin. In A, the mean value of three donors is plotted. B shows a single donor representative of at least three donors. In C, each dot represents a separate clone, with two donors shown. In D, three representative clones are shown from each donor. Error bars in A and C show SEM. Horizontal bars in C show means.