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. 2010 Feb 15;207(2):281–290. doi: 10.1084/jem.20091509

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© 2010 Crellin et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — After ex vivo expansion, LTi cell lines have low cytotoxic activity. (A) Flow cytometry was performed for intracellular perforin, granzyme B, and cell surface KIR expression on cNK cell lines and CD127⁺ LTi-like cell lines expanded ex vivo. (B) Geometric mean fluorescence intensity of intracellular granzyme B or perforin staining is shown for cNK or CD127⁺ LTi-like cell lines and LTi-like clones after ex vivo expansion. (C) K562 cells were labeled with a fluorescent dye and co-cultured with cNK or CD127⁺ LTi-like cell lines or LTi-like clones at the indicated ratios for 5 h, and cytotoxicity was determined by propidium iodide staining. Unlabeled K562 cells were added at the indicated ratios to assess nonspecific cell death (control). One of four donors analyzed is shown in A. Each dot represents a separate donor or clone in B. The mean of cell lines from two donors and three clones from a single donor are shown in C. Horizontal bars in B show the means. Error bars in B and C show SEM.