Figure 5.
CD117+CD161+CD127- iNK cells contain cNK precursors. (A) Lin−CD117+CD161+CD127− and CD117+CD161+CD127+ cells were sorted from human tonsils. (B) Isolated populations were expanded ex vivo, and expression of indicated molecules was assessed by flow cytometry. (C) RORC, IL-22, and IFN-γ mRNA after ex vivo expansion, normalized to 18S expression. A single donor representative of two is shown in A and B, and C represents the mean and SEM of at least two donors.
