Figure 4.

Endogenous androgens play a role in angiogenesis in males but not females. (A) Vascularization of Matrigel plugs harvested from male mice after 14 d, with representative images (n = 8/group; combined data from four independent experiments are shown). Sections were immunohistochemically stained for vWF (3,3′-diaminobenzidine [DAB] + nickel, eosin). Bars, 100 µm. (B) Vascularization of Matrigel plugs harvested from female mice, with representative images (n = 5–6/group; combined data from three independent experiments are shown). Sections were immunohistochemically stained for vWF (DAB, hematoxylin). Bars, 50 µm. ***, P < 0.001 versus control using ANOVA. All data are expressed as means ± SEM.