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. 2010 Feb 15;207(2):345–352. doi: 10.1084/jem.20091924

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© 2010 Sieveking et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Endogenous androgens modulate angiogenic/progenitor cell mobilization in response to ischemia. (A and B) Ulex⁺/AcLDL⁺ cells in the bone marrow (A) and spleen (B). Cells were seeded at a density of 5 × 10⁶ cells/mm² and after 4 d were assessed for the ability to ingest 4 µg/ml AcLDL (Invitrogen) and to bind 10 µg/ml FITC–UEA-1 (Sigma-Aldrich) via fluorescence microscopy. (C and D) SCA-1⁺/CXCR4⁺ cells in the bone marrow (C) and spleen (D). Hematopoietic progenitor cells were assessed using methylcellulose assays. (E and F) Granulocyte/macrophage CFUs in the bone marrow (E) and spleen (F). (G and H) Erythroid CFUs in the bone marrow (G) and spleen (H; n = 6/group; combined data from three independent experiments are shown). *, P < 0.05; **, P < 0.01; and ***, P < 0.001 using ANOVA. All data are expressed as means ± SEM.