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. Author manuscript; available in PMC: 2010 Feb 16.
Published in final edited form as: Virology. 2008 Mar 5;375(1):94. doi: 10.1016/j.virol.2007.12.044

Figure 1.

Figure 1

Inhibition of NFκB in BCBL-1 cells leads to increased lytic gene expression as well as increased apoptosis.

BCBL-1 cells were treated with the indicated amounts of Bay 11-7082 or vehicle (DMSO) for 30min at 37°C. Cells were resuspended in fresh media and total RNA and total protein were harvested at 48 hours post treatment. Taqman real time PCR was performed on cDNA made from total RNA. ddCT values (log based 2 indicator of fold change) for lytic genes Orf 50, Orf57 and gB are shown in panel (A). All values were normalized to GAPDH levels, and are expressed for each gene in BCBL-1 cells treated with Bay 1170-82 relative to those treated with the same amount of DMSO. Panel (B) shows a dose dependent increase in apoptosis as measured by PARP cleavage with increasing doses of the Bay 11-7082 compound. 20ug of total protein was blotted for PARP protein and β actin as a loading control.